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BANGKOK (AP) 鈥� Asian shares sank again on Wednesday as the latest set of U.S. tariffs, including a massive 104% levy on Chinese imports, was due to take effect.

Japan鈥檚 Nikkei 225 index initially lost nearly 4% and markets in South Korea, New Zealand and Australia also declined.

On Tuesday, the S&P 500 dropped 1.6% after wiping out an early gain of 4.1%. That took it nearly 19% below its record set in February. The Dow Jones Industrial Average dropped 0.8%, while the Nasdaq composite lost 2.1%. Uncertainty is still high about what President Donald Trump will do with his trade war.

The sharply higher tariffs were scheduled to kick in after midnight Eastern time in the U.S., and investors have no idea what to make of President Donald Trump鈥檚 trade war.

The retreat overnight and into early Wednesday in Asia followed rallies for stocks globally earlier in the day, with indexes up 6% in Tokyo, 2.5% in Paris and 1.6% in Shanghai.

The Nikkei 225 in Tokyo fell more than 3.9% before leveling off. About an hour after the market opened it was down 3.5% at 31,847.40.

South Korea’s Kospi lost 1% to 2,315.27, while the S&P/ASX 200 in Australia declined 2% to 7,359.30. Shares in New Zealand also fell.

Analysts have been warning to expect more swings up and down for financial markets given the uncertainty over how long Trump will keep the stiff tariffs on imports, which will raise prices for U.S. shoppers and slow the economy. If they last a long time, economists and investors expect them to cause a recession. If Trump lowers them through negotiations relatively quickly, the worst-case scenario might be avoided.

Hope still remains on Wall Street that negotiations may be possible, which helped drive the morning鈥檚 rally. Trump said Tuesday that a conversation with South Korea鈥檚 acting president helped them reach the 鈥渃onfines and probability of a great DEAL for both countries.鈥�

On Tuesday, Japanese stocks led global markets higher after the country鈥檚 prime minister, Shigeru Ishiba, appointed his trade negotiator for talks with the United States following a conversation with Trump.

China said it will 鈥渇ight to the end鈥� and warned of countermeasures after Trump threatened on Monday to raise his tariffs even further on the world鈥檚 second-largest economy.

White House press secretary Karoline Leavitt said Tuesday that Trump鈥檚 threats of even higher tariffs on China will become reality after midnight, when imports from China will be taxed at a stunning 104% rate.

That would coincide with Trump鈥檚 latest set of broad tariffs, which are scheduled to kick in at 12:01 a.m. And Trump has made clear that he does not intend to have any exemptions or exclusions, according to the top U.S. trade negotiator, Jamieson Greer.

The U.S. trade representative also said in testimony before a Senate committee that roughly 50 countries have already been in contact, and he鈥檚 told them: 鈥淚f you have a better idea to achieve reciprocity and to get our trade deficit down, we want to talk with you, we want to negotiate with you.鈥�

Trump鈥檚 trade war is an attack on the globalization that鈥檚 shaped the world鈥檚 economy and helped bring down prices for products on store shelves but also caused manufacturing jobs to leave for other countries. Trump has said he wants to narrow trade deficits, which measure how much more the United States imports from other countries than it sends to them as exports.

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AP Business Writers Stan Choe and Matt Ott contributed.